Faculty and research guide

Laboratory of Plant Pathology (Hirata)

Hisae Hirata

Goals of the laboratory:
In collaboration with researchers all over the world, we are analyzing on the molecular level the interaction between plants and pathogenic microorganisms. Our goal is to clarify plants’ innate mechanism for creating a resistance response to external attacks and the mechanism through which bacteria break down this response, enabling the attacker to induce infection. We are also working on creating new plants that do not succumb to infection, using genetic recombination, and this work may help to safeguard the world’s food supply.

Research and methods of analysis:
Experimental inoculation of plants against pathogenic microorganisms, experimental induction of RNA silencing in plants, expression analyses using fluorescent proteins (GFP, etc.), observations using a fluorescence microscope and a confocal laser microscope, in vivo expression analyses using a particle gun, interactive analyses involving in vitro molecules, genetic cloning, DNA sequence analyses, expression induction of proteins, sequence analyses of proteins, TOF-MS (mass spectral analysis) of proteins, microarray analyses, and real time RT-PCR, proteomics, and also, use of the latest large instruments installed in our laboratory and gene testing facility

Specific themes:
[1] Identification of the pathogenesis-related genes of phytopathogenic bacteria. Clarification of the genetic initiation mechanism in the life of pathogenic bacteria by clarifying their production control mechanisms
[2] Clarifying the mechanism that creates a resistance response in plants to phytopathogenic bacteria and the mechanisms that inhibit it
[3] Development of a quick and accurate bacteria-detection method using molecular and/or biological means, including the detection of pathology-inducing bacteria afflicting human beings