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Bombyx mori Biotechnology using Bacmid

Introduction

 After post-genome period, study on expression of human origin genes has come under the light.  Although human genome project contributed to know the roles of many genes, functions of them have not yet been elucidated.Therefore, we have been studying production of proteins of human origin in either insect cells or Bombyx mori larvae; especially, about inserting genes and their effective expression.Details are followed.

Details

1.Study on a large scale gene expression in Bombyx mori larvae using BmNPV bacmid

    カイコ個体を高次タンパク質生産工場とする昆虫バイオテクノロジー    

2. Expression of human (pro)renin receptor in silkworm larvae and pupa

    BmNPVバクミドを用いたヒトプロレニン受容体の発現   

3. The study about glycoprotein production in insect cells;

(a)Applications of chaperones in expression of glycoproteins in insect cells.

(b)Enhancement of the biological activity of glycoproteins expressed in insect cells.

   昆虫細胞を用いた糖タンパク質生産における分子生物学的研究   

4.Development of an Anti-body based assay for determination of baculoviral titers.

   抗DNA―binding proteinを用いた迅速なバキュロウイルスタイター測定法の開発   

(a)Visualization of Foreign proteins using Confocal Microscope.
(b)Study of expression of Standard protein like GFP and its effect on Cell cycle of Insect Cells.

   昆虫細胞における外来遺伝子発現     

 

 

Laboratory of Biotechnology.
Green Chemistry Research Division, Research Institute of Green Science and Technology