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Research Project

Research

 On advent of next-generation sequencer the decoding speed was improved 100–1000 times to that of the conventional one, but a lots of genetic function remains unclear. In our laboratory, Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid system was developed firstly in the world, which accelerates gene expression with rapid and simple procedure. Recently, virus-like particles were expressed and purified targeting for vaccination of infectious disease, drug delivery system, and innovative applications by fusing with nanotechnology.

From Silk Road to Bio-Road is spread the whole world from our laboratory.

We are performing Biotechnological projects as follows. 

  1. GlaxoSmithKline, Inc. (GSK Japan) Research Grant, “Display of recombinant 4a accessory protein on virus like particles of Middle Easter respiratory syndrome to understand its specificity for viral dsRNA”, (PI: Vipin Kumar Deo), 2016/12/31~2018/3/31.
  2. Grant-in-Aid for Exploratory Research (Grant No. 16K14900): Research project entitled, "Development of 4-valent-dengue vaccine using silkworm biotechnology for next-generation medical use" was funded by Ministry of Education, Culture, Sports, Science and Technology from 2016-2018 (Enoch Y. Park).
  3. Grant-in-Aid for Scientific Research (A) (Grant No. 16H02544): Research project entitled, "Basic research on vaccine development for protozoan infectious disease treatment using polyvalent virus-like particles with high immune response" was funded by Ministry of Education, Sports, Science and Technology" from 2016-2019 (Enoch Y. Park).

  4. Sasagawa Scientific Foundation, Grant (No. 28-304):"Development of influenza virus detection using chiral fluorescent carbon nanotwisted ribbon and silver nanoparticles” by Syed Rahin Ahmed, 2016.
  5. HEIWA NAKAJIMA FOUNDATION Grant: “Development of Rapid detection of Dengue virus I~IV using molecular beacon nanoprobe” by Enoch Y. Park. (April 2016–March 2017).
  6. Suzuken Memorial Foundation, “Development of Norovirus detection with rapid and high sensitivity using fuctionalized nanoparticles“ by Enoch Y. Park (December 2014–November 2015).
  7. Promoting technology transfer and innovation A-STEP (FS stage: Exploratory Research) Japan Science and Technology agency, “Creation of Virus-like particles tracking for cancer cells-Targeting for drug delivery application-“ by Enoch Y. Park (December 2014–November 2015).
  8. General Incorporated Association The Consortium of Universities in Shizuoka Research Foundation "Development of novel rapid and high sensitive Norovirus detection technology using functional nanomaterials" (From April 2014~February 2015)
  9. Japan Society of the Promotion of Science Bilateral Joint Research Projects/Seminars 2014 "Development of novel rapid and high sensitive virus detection technology" (From July 2014~June 2016)
  10. HEIWA NAKAJIMA FOUNDATION Grant: “Mass production of baculovirus free virus-like particles targeting for vaccine” (by Enoch Y, Park).
  11. Adaptable and Seamless Technology Transfer Program through Target-driven R&D of Japan Science and Technology Agency, (No. AS232Z01839E):"Development of mass production and purification of relaxin related factor in silkworm expression system targeting for improving impregnation ratio of livestock"(by Enoch Y Park)

  12. Grant-in-Aid for Exploratory Research (No.23760747):"Development of high throughput expression and purification system of human protein using silkaworm" (by Tatsuya Kato)
  13. Sasagawa Scientific Foundation, Grant (No.  23-625):"Expression and purification of the RSV-gag Virus like Particle pseudo typed with Influenza A (H1N1/PR/8/34) Hemagglutinin from Silkworms and Stable Insect cell lines. "(by Vipin Kumar Deo) 

  14. Grant-in-Aid for Exploratory Research (No. 23658286): "Research project entitled,"Development of technologies aimed at creation of humanized glycoprotein from silkworm for next-generation medical use" was funded from 2011-2013 by Ministry of Education, Culture, Sports, Science and Technology.
  15. Grant-in-Aid for Scientific Research (A)  (No. 22248009): Research project entitled, "Basic research on vaccine development for protozoan infectious disease treatment using antigen displayed baculovirus"by Ministry of Education, Sports, Science and Technology" was funded from 2010-2013 by Ministry of Education, Culture, Sports, Science and Technology.
  16. Research project entitled "Development of biomass ethanol production process using cellulase and its practical use" was funded from 2006 to 2007, as the Technical Development of Measures against Global Warming by Ministry of the Environment. We are taking part of this project for the efficient production of cellulase using cellulosic wastes. Tsukishima Co. Ltd. is our collaborator in this project.
  17. Grant-in-Aid for Scientific Research (B) (No. 19360372): "Synthesis of nanobiomaterial using silkworm larvae" was funded from 2007-2009 by Ministry of Education, Culture, Sports, Science and Technology.
  18. Research project entitled "Development of practical bio-ethanol prooduction process using cellulase" was funded in part by Ministry of the Environment Japan (2006-2007).
  19. Research project entitled "Development of biodiesel production using waste activated bleaching earth containing vegetable oils" was funded by New Energy and Industrial Technology Development Organization (2007-2008). Mizusawa Chem. Ind. Co. was our collaborator in this project.
  20. Research project entitled "Development of riboflavin production using waste vegetable oils" was funded as a Comprehensive Support Programs for Creation of Regional Innovation in Japan Science andTechnology Agency (2007-2009).
  21. Research project entitled "Development of novel bacmid and its application on mass expression of higher-order structured protein" was funded by the Program for Promotion of Basic Research Activities for Innovocative Biosciences (PROBRAIN) in Bio-oriented Technology Research Advencement Institution (BRAIN) (2005-2008). 
  22. Research project entitled "Efficient production of Biodiesel by reusing waste vegetable oil" was funded by The IWATANI NAOJI Foundation (2002).
  23. Grant-in-Aid for Scientific Research (C) (No. 13650854): "Mechanism of molphological development of mycelia and its application on the production of value-added bioptoduct" was funded by Ministry of Education, Culture, Sports, Science and Technology (2001-2002).
  24. Research project entitles "Research on biofuel conversion from waste oil" was funded by Steel Foundation for Environmental Protection Technology (2000).
  25. Research project entitles "Research on recombinant protein production from recombinant methylotroph Pichia pastoris" was funded by Research Foundation of Electrotechnology of Chubu (1997).
  26. Research project entitles "Research on recombinant protein production from baculovirus expression system" was funded by Shizuoka Educational Promotion Foundation (1997).
  27. Grant-in-Aid for Young Scientist (A)06750824: "Efficient production of recombinant protein from recombinant Bacillus subtilis by controlling nitrogen source" was funded by Ministry of Education, Culture, Sports, Science and Technology (1994).
  28. Research project entitles "Development of nutrient control for the culture of recombinant Bacillus subtilus and its application on production of value-added recombinant protein" was funded by Research Foundation of Electrotechnology of Chubu (1994).
  29. Grant-in-Aid for Scientific Research (C) (No. 05650795): "Optimization of antibiotic production process using microorganism consuming vegetable oil" was funded by Ministry of Education, Culture, Sports, Science and Technology (1993).
  30. Grant-in-Aid for Scientific Research (B) (No. 04453125): "Development of industrial recombinant cell fermentation and effective productions of gene products" was funded by Ministry of Education, Culture, Sports, Science and Technology (in part) (1992-1993).  

In progress

  1. Bombyx mori Biotechnology
  2. Reutilization of biomass resources

  

In break

  1. Study of the Filamentous Morphology of Fungus during Growth

 

Sasagawa Scientific Foundation, Grant (No. 28-304):"Development of influenza virus detection using chiral fluorescent carbon nanotwisted ribbon and silver nanoparticles” by Syed Rahin Ahmed, 2016.

Laboratory of Biotechnology.
Green Chemistry Research Division, Research Institute of Green Science and Technology